Molecular Techniques in Biology 320-ERS-1MTE
Wykład (WYK) Rok akademicki 2021/22

the same as in General (Main) part.

the same as in General (Main) part.

Lecture attendance, Short test (open and/or choice questions).

Lectures:

1. Basic techniques of protein and DNA analysis: non-invasive and invasive sampling for molecular tests, sample collection and storage methods of DNA isolation, determination of the quantity and quality of the isolated DNA.

2. Protein electrophoresis, immunological methods (mono and polyclonal antibodies), ELISA (in brief).

3. PCR reaction and its selected variants/types (RFLP-PCR, RT-PCR, Fast-PCR, Hot-Start PCR, real-time PCR and others). Optimization of PCR

reaction.

4. Analysis of SNP, AFLP, SSCP, FISH and DNA microarrays.

5. DNA sequencing by dideoxy method (Sanger sequencing).

6. Principles of Pyrosequencing, nanopore sequencing. Other Next-generation sequencers (Roche 454, Illumina, SMRT). Methods of Methylome sequencing.

7. Barcodes of living organisms (DNA barcoding), DNA metabarcoding (DNA barcoding from environmental samples).

Lecture, presentation of animations form the WEB.