| Literatura: |
Basic literature:
1. Elsa Lundanes, Leon Reubsaet, Tyge Greibrokk, Chromatography: Basic Principles, Sample Preparations and Related Methods Wiley, 2013.
2. Rudolf Bock, Reinhard Nießner, Separation Techniques in Analytical Chemistry, Walter de Gruyter GmbH & Co KG, 2023.
Additional literature:
1. E. Wołyniec, J. Karpińska, S. Łosiewska, M. Turkowicz, J. Klimczuk, A. Kojło, Determination of lipoic acid by flow-injection and high-performance liquid chromatography with chemiluminescence detection, Talanta, 96 (2012) 223-229.
2. L.K. Male, J. Glennon, Boron-doped diamond electrode: synthesis, characterization, functionalization and analytical applications, Analyst, 134 (2009) 1965-1979.
3. C.P. Sousa, et. al, Electroanalysis of pharmaceuticals on boron-doped diamond electrodes: a review, Chem. Electro. Chem., 6 (2019) 1-30.
4. Ligor M., Studzińska S., Horna A., Buszewski B., Corona-charged aerosol detection: an analytical approach. Critical Reviews in Analytical Chemistry, 2013, 43, 64-78.
5. Grembecka M., Lebiedzińska A., Szefer P. (2014) Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector. Microchemical Journal 117: 77-82.
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| Efekty uczenia się: |
Knowledge:
The graduate knows and understands:
- advanced topics in chemistry and deepens knowledge in the chosen specialization, i.e., chemical analytics (KP7_WG1),
- modern measurement techniques used in chemical analysis, explains the theoretical basis of measurement equipment (e.g., HPLC-CAD, HPLC-FLD, GC-MS, FAAS, ETAAS, ICP-MS) used in chemical testing (e.g., environmental samples, food) (KP7_WG5).
Social Competencies:
The graduate is ready to:
- critically evaluate information disseminated in the media, particularly in the field of chemistry, and understands the need for systematically reviewing the professional literature (KP7_KK1).
A written examination will be conducted to assess whether the intended learning outcomes have been achieved.
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| Zakres tematów: |
‒ High performance liquid chromatography (HPLC) with particular emphasis on modern detection systems: corona aerosol detector (CAD) and fluorescence detector (FLD) - structure, principle of operation, possibilities and limitations, examples of determined substances (e.g. compounds with natural fluorescence and subject to derivatization reactions).
‒ Chemometry in chemistry - selected problems: definition of chemometry, specificity of multidimensional data, area of interest in chemometry, division of chemometric methods. Selected examples of the application of chemometric methods to real research problems.
‒ Flame and electrothermal atomic absorption spectrometry (FAAS, ETAAS) and inductively coupled plasma mass spectrometry (ICP-MS): principle of operation and apparatus; spectral and non-spectral interferences, methods of interference elimination: the use of modifiers, background corrections, reaction and collision chambers; application of methods in chemical analysis.
‒ Electrochemistry: new materials and strategies used in the electroanalysis of compounds - boron-doped diamond electrode, preparation, characterization, possibilities of determinations and limitations, examples of determined substances. Analytical procedures to increase the sensitivity of determinations: modifications of the BDD electrode, application of surfactants in electroanalysis.
‒ Flow injection analysis (FIA) and its practical applications, types of detection (e.g. chemiluminescent) and possible applications using the principles of green analytical chemistry.
‒ Sample preparation stages for analysis. Separation of suspended matter using filtration processes. Sample storage.
‒ Isolation and enrichment of analytes using gas phase extraction, e.g. headspace analysis techniques. Types of sorbents used in solid phase extraction (SPE).
‒ Modifications of classical liquid-liquid extraction. Modern extractants: ionic liquids (IL), deep eutectic liquids (DES). Application of surface-active compounds in the process of analyte separation. Miniaturized extraction methods: liquid-liquid microextraction (LLME), dispersive liquid-liquid microextraction (DLLME), single-drop solvent microextraction (SDME), stationary phase microextraction (SPME).
‒ Membrane extraction. Solvent-free methods of sample preparation for analysis. Drying and purification of extracts.
‒ Analysis of the extracts obtained using spectrophotometric (UV-VIS) and chromatographic methods with different types of detection. Basic parameters for validation of the analytical method.
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