Microbiology

obowiązkowe

Substantive content:

Basics of microscopy. Light microscope, fluorescence microscope, phase contrast microscope. The application of microscopes in microbiology.

Simple staining. Types of dyes. Preparation technique of preparations. Microscopic observations.

Gram staining. Complex staining of mycobacteria and endospores of bacilli.

Microbiological media.

Techniques of sterilization and disinfection. Autoclave, Pasteur's oven, microwave sterilizer

Isolation of bacteria from environmental samples. Culture and growth of bacteria. Purification of bacterial cultures. Bacteria enumeration techniques.

Test I

Biochemical tests (eg. API)

Antibiotics and bacterial resistance. Susceptibility testing (disc diffusion tests, gradient strips)

Gram-positive aerobic bacteria.

Gram-negative bacteria

Anaerobes (eg Clostridium spp.)

Basics of virology (phage typing, cell cultures)

Test II

Literature:

Angela Edwards, Beatrix Fahnert, Greg Pryor, Anthony Strelkauskas, Jennifer Strelkauskas (2015) Microbiology: A Clinical Approach. Garland Science.

Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair GE (2020) Microbiology: An Introduction (13th Edition). Pearson Education.

Forms and conditions of credit:

To pass the subject Microbiology, you must pass two tests and attend lab classes (one absence is allowed).