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Molecular Techniques in Biology

Informacje ogólne

Kod przedmiotu: 320-ERS-1MTE Kod Erasmus / ISCED: (brak danych) / (brak danych)
Nazwa przedmiotu: Molecular Techniques in Biology
Jednostka: Wydział Biologii
Grupy: Program ERASMUS - Biologia
Punkty ECTS i inne: 6.00
zobacz reguły punktacji
Język prowadzenia: (brak danych)
Rodzaj przedmiotu:

obowiązkowe

Skrócony opis:

The aim of Molecular techniques in biology is to learn about traditional and modern research and analytical methods in the field of Molecular biology, to acquire the ability to use them in order to study genetic polymorphisms, determine the genotype of an individual, as well as obtain and analyze the sequence of the studied DNA fragments. On selected examples, students learn about the possibilities and limitations of particular analysis methods. This course shows the techniques of molecular biology as a powerful research tool enabling answers to fundamental questions in the field of evolutionary biology, ecology, genetics and genomics.

Pełny opis:

Lecture:

1. Basic techniques of protein and DNA analysis: non-invasive and invasive sampling for molecular tests, methods of DNA isolation, determination of the quantity and quality of the isolated DNA.

2. Protein electrophoresis, immunological methods (mono and polyclonal antibodies), ELISA.

3. PCR reaction and its variants (RFLP-PCR, RT-PCR, Fast-PCR, Hot-Start PCR, real-time PCR and others). Optimization of PCR reaction. Principles of PCR primer design, evaluation of their quality.

4. Analysis of SNP, AFLP, SSCP, FISH and DNA microarrays.

5. DNA sequencing by detoxification method.

6. Pyrosequencing, sequencing in nanopores. Next-generation sequencers (Roche 454, Illumina and others), principles of their functioning.

7. Barcodes of living organisms (DNA barcoding), DNA metabarcoding (DNA barcoding from environmental samples).

8. Comparative genomics, environmental genomics, population genomics, genetics of biocenoses. Molecular diagnostics and epidemiology.

Laboratory:

1. DNA isolation and gel electrophoresis.

a) Main rules of work in Molecular Biology Laboratory.

b) Pipetting practice.

c) DNA extraction from animals tissues.

d) Gel electrophoresis.

2. PCR - sex identification of mammals.

a) PCR-MULTIPLEX

b) Sex identification of mammals.

c) Gel electrophoresis.

3. Identification of polymorphisms in mitochondrial control region by PCR-RFLP.

a) Digestion of PCR products (fragment of mitochondrial ctr of common vole Microtus arvalis) with HaeIII (BsuI) enzyme (RFLP).

b) Simulation of digestion – NEBcutter V2.0

c) Gel electrophoresis.

4. Molecular species identification – part 1.

a) Clean-up of PCR products.

b) Sequencing reaction.

5. Molecular species identification – part 2.

a) Purification of sequencing reaction products with the ExTerminator kit (A&A Biotechnology).

b) Separation of sequencing products on a 3130 Genetic Analyzer (Applied Biosystems).

c) Results interpretation: BLAST, NCBI website.

6. Molecular species identification – part 3.

a) Working with the sequences obtained - ChromasLite and BioEdit programs.

b) Results interpretation - BLAST, NCBI website.

c) Constructing phylogenetic trees - MEGA program.

Literatura:

1. Carson S. 2019. Molecular Biology Techniques. A Classroom Laboratory Manual. Elsevier Science Publishing Co Inc

2. Rapley R. 2014. Molecular Biology and Biotechnology. Royal Society Of Chemistry

Efekty uczenia się:

1. Student explains the principles of particular methods of Molecular biology.

2. Student characterizes the advantages and disadvantages of particular methods of Molecular biology and knows their applications.

3. Student distinguishes between the methods of DNA fragment size analysis and DNA sequence analysis and is able to apply them in practice.

4. Student is aware of the research and application possibilities offered by the tools of Molecular biology.

5. Using an electrophoresis apparatus, a DNA thermocycler and a sequencer, student performs basic molecular analyzes to determine the genotype of the organism under study.

6. Student is able to work in a team and independently while performing laboratory and computer analyzes in the field of Molecular biology.

Metody i kryteria oceniania:

Lecture:

Written exam summarizing the subject: closed test.

Laboratory:

Written theory test: test and / or open-ended questions.

Zajęcia w cyklu "Rok akademicki 2020/21" (zakończony)

Okres: 2020-10-01 - 2021-06-30
Wybrany podział planu:


powiększ
zobacz plan zajęć
Typ zajęć: Laboratorium, 20 godzin więcej informacji
Wykład, 10 godzin więcej informacji
Koordynatorzy: Magdalena Czajkowska, Krzysztof Deoniziak
Prowadzący grup: Magdalena Czajkowska
Lista studentów: (nie masz dostępu)
Zaliczenie: Przedmiot - Zaliczenie na ocenę
Laboratorium - Zaliczenie na ocenę

Zajęcia w cyklu "Rok akademicki 2021/22" (w trakcie)

Okres: 2021-10-01 - 2022-06-30
Wybrany podział planu:


powiększ
zobacz plan zajęć
Typ zajęć: Laboratorium, 20 godzin więcej informacji
Wykład, 10 godzin więcej informacji
Koordynatorzy: Magdalena Czajkowska, Mirosław Ratkiewicz
Prowadzący grup: Magdalena Czajkowska, Mirosław Ratkiewicz
Lista studentów: (nie masz dostępu)
Zaliczenie: Przedmiot - Zaliczenie na ocenę
Laboratorium - Zaliczenie na ocenę
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Właścicielem praw autorskich jest Uniwersytet w Białymstoku.