Trends and methods in immunobiology

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Substantive content:

• General definitions: immunobiology, comparative immunology, immunocompetence, evolutionary trade-offs in immunobiology.

• Scientific problems studied by comparative immunology.

• Laboratory techniques applied in comparative immunology.

• General rules of collection of biological samples for immunological analyses.

• Methodology of the extraction of biological fluids from biological samples.

• Immunoenzymatic ELISA technique: general principles, types of ELISA tests, analysis of the results.

• Flow cytometry: definitions and general operation rules of a flow cytometer.

• Practical operation of a flow cytometer to assessment of blood parameters, analysis of the results.

• Semi-quantitative methods in immunobiology.

Literature:

Curtis Klaassen & John B. Watkins III, Casarett & Doull’s Essentials of Toxicology. Second Edition. The McGraw-Hill Companies, Inc, 2010.

Byung-Mu Lee & Sam Kacew & Hyung Sik Kim, Lu’s Basic Toxicology Fundamentals, Target Organs, and Risk Assessment. Seventh Edition. CRC Press, Taylor & Francis Group, 2018.

Forms and conditions of credit:

- final report and pass test from the laboratory

- attendance at the lab and lecture

- written exam (lecture part)