Current Methods Used in Chemical Analysis

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The topics of the classes concern modern methods used in chemical analysis: high-performance liquid chromatography with particular emphasis on modern detection systems (CAD, FLD), atomic absorption spectrometry with flame and electrothermal atomization (FAAS, ETAAS), mass spectrometry with inductively coupled plasma (ICP-MS), electrochemical methods, flow injection analysis (FIA). The construction of the devices, the principle of their operation, possibilities and limitations, examples of substances being determined will be discussed. The issues discussed also concern the use of chemometrics in chemical research.

The topics also concern the separation and enrichment of analytes with particular emphasis on the so-called solvent-free methods of sample preparation and miniaturized extraction techniques.

The lectures are supplemented by laboratory exercises, during which the student has the opportunity to gain practical skills in the above-mentioned methods used in chemical analysis.

Study profile - general academic

Study format - full-time

Course type - compulsory

Field: exact and natural sciences, discipline: chemical sciences

Year of study/semester - first year of second-cycle studies, summer semester

Prerequisites - Advanced Analytical Chemistry (310-CS2-1CANZ)

Number of teaching hours: lectures (30 hours), laboratories (60 hours).

Teaching methods: lecture (introductory, didactic discussion), laboratory exercises, experiment.

ECTS credits: 8.

Quantitative indicators:

Total student workload related to classes: 200h,

Student workload related to classes requiring direct teacher participation: 100h (ECTS: 4,0), including participation in lectures (30h), participation in extra-lecture classes (60h), participation in consultations/credits/exams (10h).

Preparation for classes/tests/exams (student’s own work): 100h (ECTS:4,0).

Basic literature:

1. Elsa Lundanes, Leon Reubsaet, Tyge Greibrokk, Chromatography: Basic Principles, Sample Preparations and Related Methods Wiley, 2013.

2. Rudolf Bock, Reinhard Nießner, Separation Techniques in Analytical Chemistry, Walter de Gruyter GmbH & Co KG, 2023.

Additional literature:

1. E. Wołyniec, J. Karpińska, S. Łosiewska, M. Turkowicz, J. Klimczuk, A. Kojło, Determination of lipoic acid by flow-injection and high-performance liquid chromatography with chemiluminescence detection, Talanta, 96 (2012) 223-229.

2. L.K. Male, J. Glennon, Boron-doped diamond electrode: synthesis, characterization, functionalization and analytical applications, Analyst, 134 (2009) 1965-1979.

3. C.P. Sousa, et. al, Electroanalysis of pharmaceuticals on boron-doped diamond electrodes: a review, Chem. Electro. Chem., 6 (2019) 1-30.

4. Ligor M., Studzińska S., Horna A., Buszewski B., Corona-charged aerosol detection: an analytical approach. Critical Reviews in Analytical Chemistry, 2013, 43, 64-78.

5. Grembecka M., Lebiedzińska A., Szefer P. (2014) Simultaneous separation and determination of erythritol, xylitol, sorbitol, mannitol, maltitol, fructose, glucose, sucrose and maltose in food products by high performance liquid chromatography coupled to charged aerosol detector. Microchemical Journal 117: 77-82.

KNOWLEDGE, student knows and understands:

‒ topics in chemistry that allow for an advanced explanation of methods used in chemical analysis and deepens knowledge in the field of: high-performance liquid chromatography with particular emphasis on modern detection systems, atomic absorption spectrometry with flame and electrothermal atomization, inductively coupled plasma mass spectrometry, electrochemical methods, flow injection analysis and deepens knowledge in the field of modern methods of sample preparation for analysis, including: extraction using solid adsorption beds and miniaturized techniques: liquid-liquid microextraction, solid-phase microextraction (KP7_WG1),

‒ modern measurement methods used in chemical analysis and understand their theoretical foundations and the operation of measurement equipment (including HPLC-CAD, HPLC-FLD, LC-MS/MS, ICP-MS, GC-MS, FAAS, ETAAS) used in chemical research (e.g., environmental samples, food) (KP7_WG5).

SKILLS, student is able to:

‒ use modern methods of isolating and enriching analytes, miniaturized extraction techniques, chemical tools and methods, particularly chromatographic, electrochemical, chemometric, and spectroscopic methods, to diagnose and analyze chemical problems (KP7_UW4),

‒ analyze experimental results using statistical, chemometric, and IT methods; assess data reliability, detect measurement errors, and justify the purpose and significance of the research performed in relation to other research (KP7_UW6).

SOCIAL COMPETENCES, student is ready to:

‒ critically analyze media content, particularly those related to current methods used in chemical analysis, and recognize the need to regularly review specialized literature (KP7_KK1).

Form of course credit:

- lecture: written exam with a grade. The condition for taking the exam is passing the laboratory exercises.

- laboratory: credit with a grade from the Instructor (entrance before performing the exercise). The final grade from the laboratory will be the average of the grades from the 12 exercises performed.

In accordance with the concept of universal design for people with special needs, it is possible to introduce flexible forms of credit in agreement between the lecturer and the student.

Assessment criteria in accordance with the principles set out in the Study Regulations of the University of Białystok adopted by Resolution No. 2527 of the Senate of the University of Białystok of June 26, 2019.

For this course, the use of AI systems is prohibited during written examinations and in the preparation of laboratory reports, with the exception of: language and stylistic correction of the text,and machine translation of text from or into a foreign language.